Forskolin-induced Swelling Assay (FIS)
The forskolin-induced swelling (FIS) assay is an imaging assay that, by measuring organoid swelling, measures CFTR function restoration and response to drug treatment. The FIS assay is a valuable tool in cystic fibrosis drug development and patient stratification and has shown its potential for clinical patient representation and personalised medicine for CF-subjects.
Cell Viability and Cytotoxicity Assays
HUB offers an extensive line of robust and innovative assays for determining cell viability and cytotoxicity. The assays have been developed in 3D culture conditions and can be multiplexed with imaging. Using a format of 384-well plates, up to 12,000 data points can be measured in one single assay to test the efficacy and toxicity of drugs.
HUB has developed co-culture assays between immune cells (native or engineered) with normal and/or tumour organoids to evaluate either specific tumour recognition/killing of tumour organoids compared to normal or the interplay between immune cells and epithelial cells in the context of diseases such as intestinal bowel diseases (IBD) or chronic obstructive pulmonary disease (COPD). Imaging, FACs analysis and IFNγ measurements are the main readouts for these assays.
- Gene expression
- DNA/RNA extraction
- Molecular Diagnostics
- Gene editing
- Mechanism of action studies
- HIC and IF Assays
- Co-culture with immune cells