Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Nature. 2009 May 14;459(7244):262-5. doi: 10.1038/nature07935. Epub 2009 Mar 29.
Sato T, Vries cheap jerseys RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, van Es JH, Abo A, Kujala P, Peters PJ, Clevers H.
The intestinal epithelium is the wholesale nba jerseys most cheap jerseys rapidly self-renewing wholesale nfl jerseys tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells China at the bottoms of small-intestinal crypts. Here big we describe the establishment of long-term culture conditions under which and single crypts undergo multiple crypt fission cheap mlb jerseys events, while simultanously generating villus-like epithelial domains as in which all differentiated cell types are present. Single sorted Lgr5(+) stem other cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. wholesale We conclude first that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.