Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro.

January 8, 2010

Hubrecht Organoid Technology

Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro.

Cell Stem Cell. 2010 Jan 8;6(1):25-36. doi: 10.1016/j.stem.2009.11.013.

Barker N1, Huch M, Kujala P, van de Wetering M, Snippert HJ, van Es JH, Sato T, Stange DE, Begthel H, cells van den Born M, Danenberg E, van den Brink S, Korving J, Abo A, Peters PJ, Wright N, Poulsom R, Clevers H.

 

Abstract

The study of gastric epithelial homeostasis and cancer has been hampered by the lack of stem cell markers and in JERSEYS vitro culture methods. The Wnt target gene Lgr5 A marks stem cells in the small intestine, colon, and hair follicle. Here, we investigated Lgr5 expression in the stomach and assessed the stem cell potential of the Lgr5(+ve) cells by using in vivo lineage tracing. In neonatal stomach, cancer. Lgr5 was expressed Ultra at the base of prospective corpus and pyloric glands, whereas expression in the adult was predominantly restricted to the base of mature cheap NBA jerseys pyloric glands. Lineage tracing revealed these Lgr5(+ve) cells to be self-renewing, multipotent stem cells responsible for the long-term Determining renewal of the Lgr5. gastric epithelium. With an in wholesale NFL jerseys vitro culture system, single Lgr5(+ve) cells efficiently generated long-lived organoids resembling mature pyloric epithelium. The Lgr5 stem wholesale jerseys cell marker and culture method described here will be invaluable tools for accelerating research into gastric epithelial renewal, inflammation/infection, and cancer.

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