Identification of stem cells in small intestine and colon by marker gene Lgr5.

October 7, 2007

Hubrecht Organoid Technology

Identification of stem cells in small intestine and colon by marker gene Lgr5.

Nature. 2007 Oct 25;449(7165):1003-7. Epub 2007 Oct 14.

Barker N1, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H.

Abstract

The intestinal cheap jerseys epithelium is the most rapidly self-renewing tissue in adult mammals. cheap jerseys It is currently believed that four Canos to six cheap mlb jerseys crypt stem cells reside at cheap nba jerseys the +4 position immediately above the Paneth cells in the small intestine; colon stem cells remain undefined. Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5, also known as Gpr49) was selected from a panel of intestinal Wnt target genes for its restricted crypt expression. Here, using two knock-in alleles, we reveal exclusive expression of Lgr5 in cycling columnar cells at the crypt base. In basketball addition, Lgr5 was expressed be in rare cells in several other tissues. Using an inducible Cre knock-in allele and the Rosa26-lacZ reporter strain, lineage-tracing experiments were performed cheap jerseys in adult mice. The Lgr5-positive crypt base columnar cell generated all epithelial lineages over a 60-day period, suggesting that it represents the stem cell of the small intestine and colon. The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers.

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