Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells.

October 1, 2010

Hubrecht Organoid Technology

Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells.

Cell. 2010 Oct 1;143(1):134-44. doi: 10.1016/j.cell.2010.09.016.

 

Snippert HJ1, van stomach der Flier LG, Sato T, van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H.

 

Abstract

Intestinal stem cells, characterized by high Lgr5 expression, reside between Paneth cells at the small intestinal crypt base and divide every day. We have carried out fate mapping of individual Jerseys stem cells by generating a multicolor Cre-reporter. As a population, Lgr5(hi) stem cells persist Finding life-long, yet crypts drift toward clonality within a period of 1-6 months. We have collected short- and long-term clonal tracing data of individual cheap jerseys Lgr5(hi) cells. These wholesale mlb jerseys reveal that most Lgr5(hi) cell divisions occur symmetrically and do not support as a model in which two daughter cells resulting from an Lgr5(hi) cell division adopt divergent fates (i.e., one Lgr5(hi) cell and one transit-amplifying [TA] cell per division). The cellular dynamics are consistent with wholesale nba jerseys a model in which the resident stem cells double their numbers each day and stochastically adopt in stem or TA fates. Quantitative analysis shows that stem cell turnover Afl follows a pattern of neutral drift dynamics.

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